spinach photosynthesis lab report

Share on Twitter. Watch Queue Queue. The spinach extract has a wider range of colors than the green filter. Hydrogen peroxide is harmful to plant cells in the leaf including the chloroplast. The discs were then transferred to a plastic cup with distilled water were the discs sunk to the bottom of the plastic cup. Sunlight energy   By Cheryl G11 Advanced Biology Both the spinach extract and the green filter are green in color. Copyright © 2020 IPL.org All rights reserved. Photosynthesis Lab Background The rate of photosynthesis can be measured in two different ways: the disappearance of substrate or the amount of products produced. The rate at whichphotosynthesis occurs can be based off many factors such as light intensity . This is the experiment because the one of the two tested cup with have baking soda in it which is Sodium Bicarbonate, which will produce Carbon Dioxide, which is need for photosynthesis. C6H12O6 = glucose Internal Assignment The leaves producing ATP, to reduce NADP to NADPH, and integrate CO2 to non-chemical molecular during process called carbon fixation. 9 2. We kept wrapping nylon pieces around the glass rod with the tweezers until the nylon became white and was not clear anymore. When sun lights are taken by pigments in the leaf which is chlorophyll a and b, electrons in photosystems are pushing up to high energy level. This makes sense as the Carbon Dioxide allows for the plant leaves to go through photosynthesis and produce Oxygen which in turn makes the leafs float up to the surface. Photosynthesis : the lab - discussion & conclusion. During the reaction carbon dioxide, water is converted into glucose and oxygen as shown below: Download the report sheet, complete it, and then submit it via Blackboard email as an attachment.Do not change the format.All blue shaded areas require answers. Plan your 60-minute lesson in Science or Photosynthesis with helpful tips from Mitchell Smith Plants use photosynthesis to produce their own food (glucose), some water and the oxygen that we need. Photosynthesis Lab Report. Then the discs were timed on how long it took for half of the fifteen discs to float to the surface. O2 = oxygen Hypothesis As the concentration of carbon dioxide is increased, the rate of photosynthesis is going to increase until it reaches a certain point from which the rate of photosynthesis will be constant. So what does the data say? Spinach Photosynthesis Lab Report - Google Docs. Spinach Disk Photosynthesis Lab Watch Queue Queue Without the sunlight this process and its stages wouldn’t be able to function properly to create sugar due to it being a light-dependent reaction. In the leaf-disk assay, all of the components necessary for photosynthesis are present. Preview text. Chloroplasts from spinach leaves were taken and put into tubes so one can understand the rate of photosynthesis in spinach cells at different light intensities. Tear up the spinach leaf and place a small number of spinach leaf fragments in the mortar, then add 2 ml of the solvent (60% isopropanol and 40% acetone) to the mortar. In this lab, a dye reduction technique was used. It occurs within each chloroplast in most of the green plants, and Carbon dioxide is one of the main components of this process. METHODS Pigment samples were extracted from spinach leaves by grinding and filtering the spinach leaves taken and then suspending the pigments in … Photosynthesis in Spinach Leaves and Respiration in Beans. Light Intensity’s Effect on the Rate of Photosynthesis Measured by Light Distance and Spinach Leaf Discs Abstract In the following lab, an experiment was conducted to test the effect of light intensity (measured by distance) on the rate of photosynthesis by exposing spinach leaf discs in a sodium bicarbonate-dish soap solution to a bare lamp … In testing the hypothesis we use 4 test experiments 1 with soapy water as the negative control, one with Sodium bicarbonate as the positive control, one with no light, and one with warm water and light. ... the spinach was punched and many leaf disks were made. measuring how long it took spinach leaf disks to float. Hypothesis 1: I believe that the spinach leaves that are only exposed to room light will have a slower rate of photosynthesis, and the spinach plants that are exposed to incandescent light will photosynthesis faster. This leads us to reject the null hypothesis and provides support for the alternative hypothesis. Photosynthesis is a process used by plants and specific types of bacteria in order to make their own food source from sunlight to chemical energy. The experiment found that there was a connection between the two and that the rate of photosynthesis is affected by an increased concentration of carbon dioxide. The spinach "coins" will photosynthesize in an environment that is stimulated with carbon dioxide, so the leaves in the plastic cup filled with the baking soda solution will float to the top, signaling photosynthesis. Simon Fraser University. View full document. Spinach was used during this experiment and is a member of the family of ‘Chenopodiaceae’. Assignment for the Leaf Disk Assay Lab. Using the ‘spinach immersion’ technique, the rate of photosynthesis under various conditions was quantified by observing the amount of spinach floating on top of a … Photosynthesis is the process by which plants and other photoautotrophs synthesize organic compounds from carbon dioxide (Faculty of Science and Horticulture, 2018). Photosynthesis has to take place due to the fact that it helps eukaryotes survive. The effects of Light Quality on the Rate of Photosynthesis Measure through Floating Spinach Disks relationship of different wavelengths of light and the rate of photosynthesis in spinach leafs. Date: January There are. The plant’s chloroplasts, along with water, carbon dioxide, and light are the essential elements required for photosynthesis to take place. Create your own unique website with customizable templates. Purpose Photosynthesis Lab; Respiration Lab; Enzyme Lab Photosynthesis lab. The hypothesis being tested is, If the spinach is placed in the CO2 solution, the light source will cause photosynthesis to occur and the leaves to rise. As the amount of sodium bicarbonate increased the rate of photosynthesis also increased, this is because bicarbonate provides carbon dioxide (CO2) for the photosynthetic reaction which was also found in the experiment: An Assay Utilizing Leaf Disks (Guy, 1985). This video is unavailable. It comes from a leaf, winter annual plant, which evolved in the Fertile Crescent of the Middle East. Photosynthesis I: An Assay Utilizing Leaf Disks. Introduction & Background Through this experiment, we want. Plant material will generally float in water. 12 H2O+ 6 CO2 → 6 H2O+ C6H12O6 + 6 O2 This causes an absorption of energy from red and blue wavelengths which is used in photosynthesis. 5.1.2 Reactions of glycolate in the peroxisome 2018, Accessed 16 March 2018) After recording the rates of photosynthesis, the mean was calculated by taking the rates measured and adding them together, then dividing by the number of replicants to find the average between them all. Findings fuel hopes for improved food-crop efficiency. The variation of the concentration of sodium bicarbonate will have an effect on the rate of photosynthesis of spinach (Spinacia oleracea). Purpose: The purpose of this experiment was to determine the effect of sodium bicarbonate on the rate of photosynthesis of spinach leaf disks. The overall balanced equation is: 1985. We divided the total mass of the product by the total mass of the reactant and multiplied the solution by 100. This also supports the findings of other studies that tested similar variables. The equation for photosynthesis can be written as follows: 6CO 2 + 6H 2 O + light energy → C 6 H 12 O 6 + 6O 2. University. Conclusion:  The graphical representation above show how the cup with the leafs that had the Sodium bicarbonate, which gave Carbon Dioxide for the plant to use, was able to produce Oxygen more than the one without any. The main issue that was faced was when the leaf disks were being exposed to the higher percentages of sodium bicarbonate while they were being vacuumed in the syringe. Spinach leaves vibrate to kick off photosynthesis July 16th, 2014 Posted by Nicole Casal Moore-Michigan It takes about one-third of a second to blink your eye. In the light independent reaction stage, H^+ and ATP are used to fix 〖CO〗_2 to make organic molecules. Students will write a lab report including, hypothesis, experimental design, data collection / analysis, and conclusion (findings). Fatty acid, Basically, plants store energy by the process of photosynthesis (when plants absorb energy from the sun). When completing this experiment there was one mane issue with our experiment that might have affected the results. The variation of the concentration of sodium bicarbonate will have an effect on the rate of photosynthesis of spinach (Spinacia oleracea). The “photo” part of photosynthesis is in reference to the fact that the process requires light. II. According to our data, the solution of water + sodium bicarbonate has caused the spinach leaves to begin respiration of photosynthesize causing them to rise in the presence of carbon dioxide. Photosynthesis involves two different processes, which are light dependent reaction and light independent reaction. Introduction: Glyoxylate then undergoes a transamination reaction carried out by glyoxylate glutamate aminotransferase. In this experiment, the leaves will only rise to the surface when enough CO2 and light is present to allow the leaf disks to carry out photosynthesis and produce O2. When exposed to light, the disks use carbon dioxide and water to produce oxygen and glucose. use a process called photosynthesis to covert energy from the sun into energy they can use for their activities. As photosynthesis occurs oxygen gas is produced and as this is made in the deflated spinach leaves they are then inflated to float to the top of the beaker. Where: CO2 = carbon dioxide Independant Variable: Concentration of sodium bicarbonate solution Dependent Variable: Rate of reaction Control Variables: Light intensity, volume of solution Sodium bicarbonate Electronic Scale Distilled water 30 mL syringes x 6 Stopwatch Swing arm lamp Single hole punch 40-mL The 95% confidence interval is found by taking the mean plus/minus standard deviation divided by the square root of the total number of, The variation of the concentration of sodium bicarbonate will have an effect on the rate of photosynthesis of spinach (Spinacia oleracea). Multiplied the solution by 100 sun into energy they can use for their activities on the rate at whichphotosynthesis can... The plastic cup with distilled water to change the conditions spinach photosynthesis lab report the oxidation of. This also supports the findings of other studies that tested similar variables family! Timed on how long it took for half of the fifteen discs to float to absorption. 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